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"York" Human malaria parasites in continuous culture. In vitro gametocyte production required approximately 15 days. Studies are now necessary to quantify once- infected red blood cells in patients with or without early rising asexual parasites following artemisinin-based combination treatments. Staging of asexual and sexual parasites development in peripheral blood Stages of asexual parasite development in peripheral blood were estimated as follows: Semilogarithmic plots of asexual parasitaemia versus time a in all children black line , children with green line and without blue line early rising asexual parasitaemia, and b in anaemic blue line and non-anaemic green line children treated with artesunate-amodiaquine, artemether-lumefantrine or dihydroartemisinin-piperaquine.
Giemsa-stained slides of the cultures were prepared daily to monitor parasitemia and gametocytemia [ 7 ].
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- EARLY RISING ASEXUAL PARASITAEMIA (ERAP), INITIALLY DEFINED AS 'AN INCREASE KINETICS OF RELEASE...
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"York" Table 3 Risk factors for early rising asexual parasitaemia in malarious children following artemisinin-based combination treatments. Results ERAP occurred in of children. Ring forms of varying stages were found in the peripheral blood of all children. Subscribe to Table of Contents Alerts. Several authors affirm that recently isolated strains are more likely to form gametocytes than strains that have been in culture for long periods of time [ 21 , 22 ].
This, in addition to relatively rapid clearance of parasitaemia, should lead to reduced likelihood of progression of non-severe to severe malaria in these children because severe malaria has been attributable to parasite sequestration in vital organs [ 41 , 42 ], or to release of, and imbalance between pro-inflammatory and anti-inflammatory cytokines by sequestered parasites in deep tissue [ 43 ].
Giemsa-stained slides of the cultures were prepared daily to monitor parasitemia and gametocytemia [ 4 ].
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Panel f shows only monoexponential terminal elimination half time of 1. Also, as expected, in age-and treatment-matched children with and without early rising asexual parasitaemia, parasite clearance determined by microscopy was rapid and similar in both groups. Features of children with or without early rising asexual parasitaemia matched for gender, enrolment parasitaemia, treatment and same day presentation who were treated with artesunate-amodiaquine, artemether-lumefantrine or dihydroartemisinin-piperaquine.
Parameters for disposition of asexual parasitaemia in children with or without early rising asexual parasitaemia following artemisinin-based combination treatments. Giemsa-stained slides of the cultures were prepared daily to monitor parasitemia and gametocytemia [ 4 ]. During this time, it was possible to define the specific day each gametocyte stage appeared and their maturation from early young gametocyte to late mature gametocyte stages.
Chavalitshewinkoon-Petmitr Protocol Cultures treated with two serial sorbitol treatments were subcultured at 0. "York"